Protocols

ES cells

• preparation for aggragation with embryos
• electroporation
• freezing

In vitro differentiation of ES cells into:

• cardiac muscle
• neuronal lineages
• cystic embryoid bodies

In vitro organ cultures

• kidney
• lungs
• limb

Gene expression detections in embryos

• whole mount lacZ staining
• whole mount human placental alkaline phosphatase staining
• whole mount immunostaining
• whole mount RNA in situ hybridization

Other preparations

• skeletal staining/preparation