Hands-on course in the Tetraploid Embryo Complementation assay October 29 - 31, 2008
We are pleased to announce an opportunity for you to further your embryo manipulation skills by learning the tricks of the trade of tetraploid embryo aggregation. This hands-on course will be held on the days following the TT2008 meeting. (Wed 29 - Fri 31).
The course will contain all aspects of the technique, including electro-fusion of 2-cell stage mouse embryos, preparation of ES cells, zona removal, and aggregation.
Course outline Wed Oct 29 13.30 - 14.30 Introduction by Andras Nagy. Presentation of the history, background and technique, questions and discussion. 89 Chestnut, TT2008 venue Thu Oct 30, 09.00 - 17.00 Course start, changing to scrubs, entering the Transgenic Core Lab at 9am 25 Orde street, TCP Preparation of embryo culture plates Demo and hands-on practice Collection of E1.5 embryos Demo and hands-on practice Electro-fusion of E1.5 embryos Demo and hands-on practice Passage of ES cells for next day aggregation Demo Preparation of aggregation plates Demo and hands-on practice Fri Oct 31, 09.00 - 17.00 Course continuation, changing to scrubs, entering the Transgenic Core Lab at 9am 25 Orde street, TCP Tour of TCP Removalof zona pellucida Demo and hands-on practice Preparation of ES cells for aggregation Demo Aggregation of tetraploid embryos and ES cells Demo and hands-on practice Fri Oct 31 Observation of aggregates Optional 25 Orde street, TCP
Lunch will be served both days.
This course is now full. We can unfortunately not accept any more participants.
Last updated Aug 14, 2008
