U of TLTRI Mount Sinai publications
Dept of BiochemDept of Molecular Genetics





Contact Info


For the most recent publication listing, please visit the PubMed lab listing


Selected publications:

Lavoie H, Sahmi M, Maisonneuve P, Marullo SA, Thevakumaran N, Jin T, Kurinov I, Sicheri F, Therrien M. MEK drives BRAF activation through allosteric control of KSR proteins. (2018) Nature. 554(7693):549-553.

Daou S, Sicheri F. Vivid views of the PINK1 protein. (2017) Nature. 552(7683):38-39.

Keszei AF, Sicheri F. Mechanism of catalysis, E2 recognition, and autoinhibition for the IpaH family of bacterial E3 ubiquitin ligases. (2017) Proc Natl Acad Sci U S A. 114(6):1311-1316.

Wan LC, Maisonneuve P, Szilard RK, Lambert JP, Ng TF, Manczyk N, Huang H, Laister R, Caudy AA, Gingras AC, Durocher D, Sicheri F. Proteomic analysis of the human KEOPS complex identifies C14ORF142 as a core subunit homologous to yeast Gon7. (2017) Nucleic Acids Res. 45(2):805-817.

Wilson MD, Benlekbir S, Fradet-Turcotte A, Sherker A, Julien JP, McEwan A, Noordermeer SM, Sicheri F, Rubinstein JL, Durocher D. The structural basis of modified nucleosome recognition by 53BP1. (2016) Nature. 536(7614):100-3.

Wan LC, Pillon MC, Thevakumaran N, Sun Y, Chakrabartty A, Guarné A, Kurinov I, Durocher D, Sicheri F. Structural and functional characterization of KEOPS dimerization by Pcc1 and its role in t6A biosynthesis. (2016) Nucleic Acids Res. 44(14):6971-80.

Zeqiraj E, Tian L, Piggott CA, Pillon MC, Duffy NM, Ceccarelli DF, Keszei AF, Lorenzen K, Kurinov I, Orlicky S, Gish GD, Heck AJ, Guarné A, Greenberg RA, Sicheri F. Higher-Order Assembly of BRCC36-KIAA0157 Is Required for DUB Activity and Biological Function. (2015) Mol Cell. 59(6):970-83.

Li JJ, Cao C, Fixsen SM, Young JM, Ono C, Bando H, Elde NC, Katsuma S, Dever TE, Sicheri F. Baculovirus protein PK2 subverts eIF2α kinase function by mimicry of its kinase domain C-lobe. (2015) Proc Natl Acad Sci U S A. 112(32):E4364-73

Thevakumaran N, Lavoie H, Critton D,Tebben A, Marinier A, Sicheri F, Therrien M. Crystal structure of a BRAF kinase domain monomer explains basis for allosteric regulation. (2015) Nat Struct Mol Biol. 22, 37-43.

Sanches M, Duffy NM, Talukdar M, Thevakumaran N, Chiovitti D, Canny M, Lee K, Kourinov I, Uehling D, Al-Awar R, Poda G, Prakesch M, Wilson B, Tam V, Schweitzer C, Toro A, Lucas JL, Vuga D, Durocher D, Qingping Z, Patterson JB, Sicheri F. Structure and mechanism of action of the hydroxyl aryl aldehyde class of IRE1 endoribonuclease inhibitors. (2014) Nature Communications, 5, 4202

Zeqiraj E, Tang X, Hunter RW, Garcia-Rocha M, Judd A, Deak M, von Wiamowitz-Moellendorff A, Kurinov I, Guinovart JJ, Tyers M, Sakamoto K, Sicheri F. Structural basis for the recruitment of glycogen synthase by glycogenin. (2014) Proc Natl Acad Sci, 111, E2831-40

Huang H, Zeqiraj E, Dong B, Jha BK, Duffy NM, Orlicky S, Thevakumaran N, Talukdar M, Pillon MC, Ceccarelli DF, Wan LC, Juang YC, Mao DY, Gaughan C, Brinton MA, Perelygin AA, Kourinov I, Guarne A, Silverman RH, Sicheri F. Dimeric Structure of Pseudokinase RNase L Bound to 2-5A Reveals a Basis for Interferon-Induced Antiviral Activity. (2014) Mol Cell, 53, 221-34

Huang H, Ceccarelli DF, Orlicky S, St-Cyr DJ, Ziemba A, Garg P, Plamondon S, Auer M, Sidhu S, Marinier A, Kleiger G, Tyers M, Sicheri F. E2 enzyme inhibition by stabilization of a low-affinity interface with ubiquitin. (2014) Nat Chem Biol, 10, 156-63

Fradet-Turcotte A, Canny MD, Escribano-Diaz C, Orthwein A, Leung CC, Huang H, Landry MC, Kitevski-LeBlanc J, Noordermeer SM, Sicheri F, Durocher D. 53BP1 is a reader of the DNA-damage-induced H2A Lys 15 ubiquitin mark. (2013) Nature, 499, 50-54

Rivkin E, Almeida SM, Ceccarelli DF, Juang YC, MacLean TA, Srikumar T, Huang H, Dunham WH, Fukumure R, Xie G, Gondo Y, Raught B, Gingras AC, Sicheri F, Cordes SP. The linear ubiquitin-specific deubiquitinase gumby regulates angiogenesis. (2013) Nature, 498, 318-324

Lavoie H, Thevakumaran n, Gavory G, Li JJ, Padeganeh A, Guiral S, Duchaine J, Mao DY, Bouvier M, Sicheri F, Therrien M. Inhibitors that stabilize a closed RAF kinase domain conformation induce dimerization. (2013) Nat Chem Biol, 9, 428-436

Ernst A, Avvakumov G, Tong J, Fan Y, Zhao Y, Alberts P, Persaud A, Walker JR, Neculai A-M, Neculai D, Vorobyov A, Garg P, Beatty l, Chan P-K, Juang Y-C, Landry M-C, Yeh C, Zeqiraj E, Karamboulas K, Allali-Hassani A, Vedade M, Tyers M, Moffat J, Sicheri F, Pelletier L, Durocher D, Raught B, Rotin D, Yang J, Moran MF, Dhe-Paganon S, Sidhu SS. A strategy for Modulation of Enzymes in the Ubiquitin System. (2013) Science, 339, 590-595

Juang Y-C, Landry M-C, Sanches M, Vittal V, Charles L, Ceccarelli D, Mateo A-RF, Pruneda JN, Mao D, Szilard RK, Orlicky S, Munro M, Brzovic PS, Klevit RE, Sicheri, F, Durocher D. OTUB1 co-opts Lys48-linked ubiquitin recognition to suppress E2 enzyme function (2012) Mol Cell, 45, 384-97

Guettler S, Larose J, Petsalaki E, Gish G, Scotter A, Pawson T, Rottapel R, Sicheri F. Structural basis and sequence rules for substrate recognition by Tankyrase explain the basis for cherubism disease.  (2011) Cell, 147, 1340-54.

Ceccarelli DF, Tang X, Pelletier B, Orlicky S, Xie W, Plantevin V, Neculai D, Chou YC, Ogunjimi A, Al-Hakim A, Varelas X, Koszela J, Wasney GA, Vedadi M, Dhe-Paganon S, Cox S, Xu S, Lopez-Girona A, Mercurio F, Wrana J, Durocher D, Meloche S, Webb DR, Tyers M, Sicheri F. An Allosteric Inhibitor of the Human Cdc34 Ubiquitin Cojugating Enzyme. (2011) Cell, 145, 1075-87.

Nakada S, Tai I, Panier S, Al-Hakim A, Iemura S, Juang YC, O'Donnell L, Kumakubo A, Munro M, Sicheri F, Gingras AC, Natsume T, Suda T, Durocher D. Non-cononical inhibition of DNA damage-dependent ubiquitination by OTUB1.(2010) Nature, 466, 941-6.

Orlicky S, Tang X, Neduva V, Elowe N, Brown ED, Sicheri F, Tyers M. An allosteric inhibitor of substrate recognition by the SCF(Cdc4) ubiquitin ligase. (2010) Nat Biotechnol, 28, 733-7.

Wiseman RL, Zhang Y, Lee KP, Harding HP, Haynes CM, Price J, Sicheri F, Ron D. Flavonol activation defines an unanticipated ligand-binding site in the kinase-RNase domain of IRE1. (2010) Mol Cell, 38, 291-304.

Rajakulendran T, Sahmi M, Lefrançois M, Sicheri F, Therrien M. A dimerization-dependent allosteric mechanism drives RAF catalytic activation. (2009) Nature, 461, 542-5.

Mao DYL, Neculai D, Downey M, Orlicky S, Haffani Y, Ceccarelli DF, Ho JSL, Szilard RK, Zhang W, Ho CS, Wan L, Fares C, Rumpel S, Kurinov I, Durocher D, Sicheri F. Atomic structure of the KEOPS complex: an ancient protein kinase-containing molecular machine. (2008) Molecular Cell, 32, 259-75.

Rajakulendran T, Sahmi M, Kourinov I, Tyers M, Therrien M, Sicheri F. CNK and HYP form a discrete dimer via their SAM domains to mediate RAF kinase signaling. (2008) Proc Natl Acad Sci, 105, 2836-41.

Kurz T, Chou Y-C, Willems A, Meyer-Schaller N, Hecht M-L, Tyers M, Peter M, Sicheri F. Dcn1 Functions as a Scaffold-Type E3 Ligase for Cullin Neddylation (2008) Molecular Cell, 29, 23-35.

Lee K, Dey M, Neculai D, Cao C, Dever TE, Sicheri F. Structure of the Dual Enzyme Ire1 Reveals the Basis of Catalysis and Regulation in Nonconventional RNA Splicing. (2008) Cell, 132, 89-100.

Wiesner S, Ogunjimi AA, Wang HR, Rotin D, Sicheri F, Wrana JL, Forman-Kay JD. Autoinhibition of the HECT-Type Ubiquitin Ligase Smurf2 through Its C2 Domain. (2007) Cell, 30, 651-62.

Tang X, Orlicky S, Lin Z, Willems A, Neculai D, Ceccarelli DF,  Mercurio F, Shilton BH, Sicheri F*, Tyers M. Suprafacial orientation of the SCFCdc4 dimer accommodates multiple geometries for substrate ubiquitination. (2007) Cell, 129, 1165-1176.

Wiesner S, Wybenga-Groot LE, Warner N, Lin H, Pawson T, Forman-Kay JD, Sicheri F. A change in conformational dynamics underlies the activation of Eph receptor tyrosine kinases. (2006) EMBO J., 25, 4686-4696.  

Aviv T, Lin Z, Ben-Ari G, Smibert CA, Sicheri F. Sequence-specific recognition of RNA hairpins by the SAM domain of Vts1p. (2006) Nat Struct Mol Biol, 13, 168-176.  

Dar AC, Dever TE, Sicheri F. Higher-order substrate recognition of eIF2α by the RNA-dependent protein kinase PKR. (2005) Cell, 122, 887-900.

Dey M, Cao C, Dar AC, Tamura T, Ozato K, Sicheri F, Dever TE.  Mechanistic link between PKR dimerization, autophosphorylation, and eIF2α substrate recognition. (2005) Cell, 122, 901-913.

Ogunjimi AA, Briant DJ, Pece-Barbara N, Le Roy C, Di Guglielmo GM, Kavsak P, Rasmussen RK, Seet BT, Sicheri F*, Wrana JL. Regulation of Smurf2 ubiquitin ligase activity by anchoring the E2 to the HECT domain. (2005). Mol Cell, 19, 297-308.

Hoang QQ, Sicheri F, Howard AJ, Yang DS. Bone recognition mechanism of porcine osteocalcin from crystal structure, (2003) Nature, 425, 977-980.

Aviv T, Lin Z, Lau S, Rendl LM, Sicheri F*, Smibert CA. The RNA-binding SAM domain of smaug defines a new family of post-transcriptional regulators. (2003) Nat Struct Biol, 10, 614-621.

Orlicky S, Tang X, Willems A, Tyers M, Sicheri F. Structural basis for phosphodependent substrate selection and orientation by the SCF (Cdc4) ubiquitin ligase. (2003) Cell, 112, 243-256.

Leung GC, Hudson JW, Kozarova A, Davidson A, Dennis JW, Sicheri F.  The Sak polo-box comprises a structural domain sufficient for mitotic subcellular localization. (2002) Nat Struct Biol,  9, 719-724.

Dar A, Sicheri, F. X-Ray Crystal Structure and Functional Analysis of Vaccinia Virus K3L Reveals Molecular Determinants for PKR Subversion and Substrate Recognition. (2002) Mol Cell, 10, 295-305.

Nash P, Tang X, Orlicky S, Chen Q, Gertler FB, Mendenhall MD, Sicheri F, Pawson T, Tyers M.  Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication. (2001) Nature, 414, 514-521.

Wybenga-Groot LE, Baskin B, Pawson T, Sicheri F. Structural basis for autoinhibition of the EphB2 receptor tyrosinekinase by the unphosphorylated juxtamembrane region. (2001) Cell, 106, 745-757.

Schindler T, Sicheri F, Pico A, Gazit A, Levitzki A, Kuriyan J. Crystal structure of Hck in complex with a Src family-selective tyrosine kinase inhibitor. (1999) Mol Cell, 3, 639-648.

Stapleton D, Balan I, Pawson T, Sicheri F. The crystal structure of an Eph receptor SAM domain reveals a mechanism for modular dimerization. (1999) Nature Structural Biology, 1, 44-49.

Sicheri F, Moarefi I, Kuriyan J. Crystal structure of the Src family tyrosine kinase Hck. (1997) Nature, 385, 602-610.

Moarefi H, LaFevre-Bernt M, Sicheri F, Huse M, Lee C-H, Kuriyan J, Miller WT.  Activation of the Src-family tyrosine kinase Hck by SH3 domain displacement. (1997)  Nature, 385, 650-653.

Sicheri F, Yang, DS-C.  Ice binding structure and mechanism of an antifreeze protein from winter flounder. (1995) Nature, 375, 427-431.